ISOLATION AND SEQUENCING OF CDNA CLONES HOMOLOGOUS TO THE V-FGR ONCOGENE FROM A HUMAN LYMPHOCYTE-B CELL-LINE, IM-9

  • 1 January 1987
    • journal article
    • research article
    • Vol. 1  (3) , 301-304
Abstract
Two c-fgr cDNA clones were isolated from a cDNA library derived from a human B lymphocyte cell line, IM-9. Sequence analysis of the clones showed that they contained inserts corresponding to nearly full-length human c-fgr mRNA, which could encode a polypeptide of 529 amino acids with a calculated molecular weight of 59478. Although the amino acid sequence between Gly-78 amino acid sequence between Gly-78 and the carboxy-terminus of the c-fgr is highly homologous to the corresponding sequence of the c-yes protein, the homology between the two proteins is low in the amino-terminal proximal region. Northern blot hybridizaton analysis using the c-fgr specific sequence showed that the c-fgr mRNA was expressed at higher level in the liver than in the brain, lung, or kidney of a human fetus.