Quantitative extraction of amphotericin B from serum and its determination by high-pressure liquid chromatography
- 1 September 1984
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 26 (3) , 314-317
- https://doi.org/10.1128/aac.26.3.314
Abstract
Therapeutic concentrations of amphotericin B in serum were measured by reversed-phase liquid chromatography with detection at 386 nm. Complete recovery of the drug and the internal standard from a 1-ml serum sample was achieved by pretreating the sample with guanidine hydrochloride and extracting it with a disposable reversed-phase phenyl extraction column. The method was sensitive to 0.005 micrograms of amphotericin B per ml and linear to at least 5 micrograms/ml. Coefficients of variation were 4.8% within-run and 6.3% between-run.This publication has 8 references indexed in Scilit:
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