ON THE ISOLATION AND GROWTH OF INDIVIDUAL MICROBIAL CELLS FROM SOIL

Publisher Website
Google Scholar
Abstract
A technique is described for the direct isolation and growth of individual vitally stained microorganisms which have been observed in soil preparations. Soil is stained with acridine orange and mounted on agar so that individual cells can be picked from the soil with a micromanipulation tool while the organism is being viewed with an ultraviolet fluorescence microscope. These cells are then grown in nutritive media to provide cultures for identification of the organisms and for studies of their nutritional, physiological, and morphological characteristics.