Computer Averaging of Single Molecules of α2-Macroglobulin and the α2-Macroglobulin/Trypsin Complex

Abstract
From electron micrographs single molecules of human .alpha.2-macroglobulin (.alpha.2M) in the closed form, the open form and as the trypsin complex were computer averaged. The molecular images are discussed. Molecules of the electrophoretically fast migrating F-form have the closed form. In the case of the .alpha.2M/trypsin complex the 2 attached trypsin molecules are located very near to each other and in the central part of the .alpha.2M molecule.