Determination of D-Penicillamine and Its Metabolites in Blood and Urine

Abstract
The time courses of concentrations of D-penicillamine (PA) and its metabolites PA disulfide [PSSP], PA-cysteine [PSSC], S-methyl PA [MeSP] in the blood and urine of rat, dog and man were determined by using a high-performance liquid chromatography with a voltammetric detector (LC-VMD) and an amino acid analyzer. The blood level of PA was highest 2 h after the oral administration of PA, and the man metabolite was PSSC in all animals. Biological half-lives (T12) of PA were 0.83 h (rat), 0.71 h (dog) and 1.13 h (men). The main metabolites in the urine were PSSC, PSSC in dog and PSSC in men.