Detection of HIV‐1 DNA in Leukocytes Using a Commercially Available Assay
- 1 January 1994
- journal article
- Published by Wiley in Vox Sanguinis
- Vol. 66 (1) , 78-80
- https://doi.org/10.1111/j.1423-0410.1994.tb00282.x
Abstract
Recently, an assay for detection of proviral HIV-1 DNA in leukocytes became commercially available. This assay (Amplicor HIV-1 test, Roche Diagnostic Systems) multiplies HIV-1DNA up to a detectable level, using the polymerase chain reaction. We studied performance of this assay on 74 samples from HIV-1-infected patients and on 41 samples from healthy blood donors. Twice a negative control sample appeared to be erroneously reactive. However, sensitivity and specificity on the patient and donor samples both were 100%. To avoid false-positive results, we advise to repeat initially reactive samples if no other data confirm HIV-infectioKeywords
This publication has 2 references indexed in Scilit:
- Reliability of polymerase chain reaction for detection of hepatitis C virusThe Lancet, 1993
- The Immunopathogenesis of Human Immunodeficiency Virus InfectionNew England Journal of Medicine, 1993