Behavioral and electrophysiological studies with live larvae and larval rinses of the red imported fire ant,Solenopsis invicta Buren (Hymenoptera: Formicidae)

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Abstract
Behavioral and electrophysiological studies with live intact larvae and larval rinses of the red imported fire ant,Solenopsis invicta Buren, give undeniable evidence of a volatile material associated with the larvae of the ant that is capable of eliciting a response from brood-tending workers. In a Y-tube bioassay, worker ants were attracted equally to an airstream blown over sibling larvae or heterocolonial larvae. Workers were also attracted to a rinse of the larvae in a spot bioassay, aggregated about a piece of surrogate brood in another bioassay, and retrieved surrogate brood treated with the rinse material. A dose-response curve constructed from electroantennograms of workers revealed a receptor response of 1–100 brood equivalents.