Bullous pemphigoid and antinuclear antibodies. Unmasking of basement membrane fluorescence in sodium chloride-treated substrates
- 1 January 1982
- journal article
- research article
- Published by American Medical Association (AMA) in Archives of Dermatology
- Vol. 118 (1) , 37-39
- https://doi.org/10.1001/archderm.118.1.37
Abstract
Immunofluorescent (IF) microscopic studies were done on a patient with bullous pemphigoid who had a high titer of antinuclear antibodies (ANA). Direct IF microscopy showed deposition of IgG and C3 [complement component 3] at the basement membrane zone (BMZ), while, by indirect IF microscopy, ANA (but not circulating pemphigoid antibodies) were detected. The 2M sodium chloride (NaCl) solution-treated substrates, in which nuclear antigens were eluted uncovered pemphigoid antibodies. Treatment of the substrates with NaCl solution unmasked BMZ fluorescence in another set of experiments in which a large amount of serum samples containing ANA was added to serum samples containing only pemphigoid antibodies. Results suggest that pemphigoid antibodies are able to react to the BMZ in the presence of concomitant ANA unless ANA bind to the nuclear antigens.Keywords
This publication has 1 reference indexed in Scilit:
- IMMUNOLOGICAL STUDIES CONCERNING THE NEPHRITIS OF SYSTEMIC LUPUS ERYTHEMATOSUSThe Journal of Experimental Medicine, 1967