Agonistic and Antagonistic Action of AP2, Msx2, Pax6, Prox1 and Six3 in the Regulation of Sox2 Expression
- 21 October 2005
- journal article
- Published by S. Karger AG in Ophthalmic Research
- Vol. 37 (6) , 301-309
- https://doi.org/10.1159/000087774
Abstract
Sox2 transcription factor is expressed in neural tissues and sensory epithelia from the early stages of development. Particularly, it is known to activate crystallin gene expression and to be involved in differentiation of lens and neural tissues. However, its place in the signaling cascade is not well understood. Here, we report about the response of its promoter to the presence of other transcription factors, AP2alpha, Msx2, Pax6, Prox1 and Six3, in a transient reporter gene assay using HEK293 cells as recipient cells. Taking our data together, AP2, Pax6 and PROX1 can activate the Sox2 promoter. Msx2 has an inhibitory effect, whereas Six3 does not affect the Sox2 promoter. These data indicate a common activating cascade at least for AP2, Pax6, Prox1 and Sox2.Keywords
This publication has 25 references indexed in Scilit:
- Transcriptional Regulation of Mouse αB- and γF-Crystallin Genes in Lens: Opposite Promoter-specific Interactions Between Pax6 and Large Maf Transcription FactorsJournal of Molecular Biology, 2004
- Functional Analysis of Chicken Sox2 Enhancers Highlights an Array of Diverse Regulatory Elements that Are Conserved in MammalsDevelopmental Cell, 2003
- Multipotent cell lineages in early mouse development depend on SOX2 functionGenes & Development, 2003
- Identification of Sox-2 regulatory region which is under the control of Oct-3/4-Sox-2 complexNucleic Acids Research, 2002
- Regulation of the Human SIX3 Gene PromoterBiochemical and Biophysical Research Communications, 2001
- Pax6 and SOX2 form a co-DNA-binding partner complex that regulates initiation of lens developmentGenes & Development, 2001
- Superactivation of Pax6-mediated Transactivation from Paired Domain-binding Sites by DNA-independent Recruitment of Different Homeodomain ProteinsJournal of Biological Chemistry, 2001
- Characterization of the MouseProx1GeneBiochemical and Biophysical Research Communications, 1998
- The Cryner Element in the Murine γ-Crystallin Promoters Interacts with Lens ProteinsOphthalmic Research, 1997
- The molecular basis of Boston-type craniosynostosis: the Pro148-->His mutation in the N-terminal arm of the MSX2 homeodomain stabilizes DNA binding without altering nucleotide sequence preferencesHuman Molecular Genetics, 1996