Expression cloning of a Na(+)-independent neutral amino acid transporter from rat kidney.
- 1 January 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (1) , 1-5
- https://doi.org/10.1073/pnas.89.1.1
Abstract
Uptake of long-chain and aromatic neutral amino acids into cells is known to be catalyzed by the Na(+)-independent system L transporter, which is ubiquitous in animal cells and tissues. We have used a Xenopus oocyte expression system to clone the cDNA of a system L transporter from a rat kidney cDNA library. The 2.3-kilobase cDNA codes for a protein of 683 amino acids. The transporter has four putative membrane-spanning domains and bears no sequence or structural homology to any known animal or bacterial transporter. When transcribed and expressed in Xenopus oocytes, the transporter exhibits many, but not all, of the characteristics of L-system transporters, suggesting that this represents one of several related L-system transporters.Keywords
This publication has 29 references indexed in Scilit:
- Transport of cationic amino acids by the mouse ecotropic retrovirus receptorNature, 1991
- STRUCTURE AND FUNCTION OF HEXOSE TRANSPORTERSAnnual Review of Biochemistry, 1991
- Expression cloning of a cocaine-and antidepressant-sensitive human noradrenaline transporterNature, 1991
- Cloning by functional expression of a member of the glutamate receptor familyNature, 1989
- Partial purification of alanine carrier from rabbit small intestine brush border membrane and its functional reconstitution into proteoliposomesBiochemical and Biophysical Research Communications, 1988
- Kinetics of Neutral Amino Acid Transport Across the Blood‐Brain BarrierJournal of Neurochemistry, 1987
- Evidence for a single common Na+-dependent transport system for alanine, glutamine, leucine and phenylalanine in brush-border membrane vesicles from bovine kidneyBiochimica et Biophysica Acta (BBA) - Biomembranes, 1987
- Patterns of Amino Acids near Signal‐Sequence Cleavage SitesEuropean Journal of Biochemistry, 1983
- Multiple transport pathways for neutral amino acids in rabbit jejunal brush border vesiclesThe Journal of Membrane Biology, 1982
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982