Enzymatic Synthesis of Galactosylkojic Acid with Immobilizedβ-Galactosidase fromBacillus circulans

Abstract
Galactosylkojic acid, in which the hydroxymethyl group of the kojic acid moiety is β-galactosylated, was synthesized by using a transgalactosidation reaction with an immobilized β-galactosidase from Bacillus circulans. With 5% kojic acid and 20% lactose as the substrates, the yield with respect to the initial amount of kojic acid was around 24% as a maximum. The inhibitory effect of galactosylkojic acid towards the oxidation reaction of L-β-(3,4-dihydroxyphenyl)alanine (L-DOPA) with tyrosinase was similar to that of kojic acid.

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