Distribution of Se75AND S35in intracellular fractions of rat liver and rat kidney

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Abstract
Groups of rats injected with Se75-labelled sodium selenite were killed at intervals of up to 2 hr and various organs and tissues were examined for Se75. The livers and kidneys contained greater concentrations of Se71i than other organs examined. Counts for blood fell to a minimum at 40 min followed by a gradual increase. The pattern for the liver was the reverse of that found for blood. The soluble fraction of the sub-cellular components isolated from liver contained the highest concentration of Se75 per mg of protein. No other component of liver appeared to play a significant role in the incorporation of Se75, into the liver cell during the examination period. The light microsomes of kidney, with a peak Se75 activity at 40 min, had a greater Se75; concentration than did other kidney fractions. Twenty minutes after the simultaneous injection of S75-labelled sodium sulphate and Se75-labelled sodium selenite approximately 90% of the Se75 in the liver and kidney sub-cellular components was proteinbound, but the major proportion of the S35, with the exception of that in the kidney light microsomes, was readily removed by dialysis.