Structural Studies on a Glucose-containing Polysaccharide Obtained from Micrococcus lysodeikticus Cell Walls

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Abstract
An acidic polysaccharide from the cell walls of Micrococcus lysodetkticus was subjected to controlled acid hydrolysis and the products were chromatographed on a Dowex 1×4 column (acetate form). Four peaks were obtained and the main one was purified further on a Bio Gel P2 column. To determine its structure, the material in this fraction was subjected to peracetylation and borohydride reduction, then its carboxyl group was esterified with diazomethane and the product was reduced with borohydride. The resulting material was found to contain nearly equimolar amounts of N-acetyl-mannosamine and glucitol. It yielded gentiobiitol on deacetylation with anhydrous hydrazine and deamination with nitrous acid. Thus there was a β-1, 6 linkage between mannosaminuronic acid and glucose in the isolated disaccharide. After N-acetylation of purified material from a partial hydrolysate of the polysaccharide, a microcrystalline disaccharide was obtained as a strontium salt.

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