Human Leucocyte Response to Migration Inhibitory Activity from Lymphocytes

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Abstract
Human lymphokines can elicit several effects associated with inflammation, e.g., leukocyte migration inhibition and fibrinolysis. These effects can be assessed in vitro by the leukocyte migration agarose technique (LMAT) and the leukocyte migration fibrinolysis technique (LMFT). The present study shows that preincubation of normal leukocytes with aprotinin, tranexamic acid and phenyl-methyl-sulfonylfluoride (PMSF) reduces or abolishes their migration inhibition response to leukocyte migration inhibition factor. The compounds exert this effect at non-toxic concentrations, which do not otherwise interfere with migration or fibrinolysis, and are non-toxic as estimated by PHA [phytohemagglutinin] stimulation of lymphocytes. The LMFT is more sensitive to the modifying effect than the LMAT. The effect of aprotinin and tranexamic acid is reversible; the effect of PMSF is irreversible.