Fine Specificity of Serum Antibodies toPlasmodium falciparumMerozoite Surface Protein, PfMSP-119, Predicts Protection from Malaria Infection and High-Density Parasitemia
Open Access
- 1 March 2004
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 72 (3) , 1557-1567
- https://doi.org/10.1128/iai.72.3.1557-1567.2004
Abstract
Antibodies to the C terminus of thePlasmodium falciparummerozoite surface protein, PfMSP-119, may inhibit merozoite invasion or block the effects of inhibitory antibodies. Here, using a competition enzyme-linked immunosorbent assay and antibody binding to wild-type and mutated recombinant proteins, we show that there are marked variations between individuals in the fine specificity of naturally acquired anti-MSP-119antibodies. Furthermore, although neither the prevalence nor the concentration of total anti-MSP-119antibodies was associated with resistance to malaria in African children, significant associations were observed between antibody fine specificity and subsequent risk of infection and high-density parasitemia during a follow-up period. Thus, the fine specificity of naturally acquired human anti-MSP-119antibodies is crucial in determining their function. Future field studies, including the evaluation of PfMSP-1 vaccine trials, should include assays that explore antibody fine specificity as well as titer.Keywords
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