Molecular imaging of gene expression in living subjects by spliceosome-mediated RNA trans-splicing
- 25 May 2004
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 101 (23) , 8693-8698
- https://doi.org/10.1073/pnas.0402772101
Abstract
Spliceosome-mediated RNA trans-splicing (SMaRT) provides an effective means to reprogram mRNAs and the proteins they encode. SMaRT technology has a broad range of applications, including RNA repair and molecular imaging, each governed by the nature of the sequences delivered by the pre-trans-splicing molecule. Here, we show the ability of SMaRT to optically image the expression of an exogenous gene at the level of pre-mRNA splicing in cells and living animals. Because of the modular design of pre-trans-splicing molecules, there is great potential to employ SMaRT to image the expression of any arbitrary gene of interest in living subjects. In this report, we describe a model system that demonstrates the feasibility of imaging gene expression by transsplicing in small animals. This represents a previously undescribed approach to molecular imaging of mRNA levels in living subjects.Keywords
This publication has 16 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Phenotype correction of hemophilia A mice by spliceosome-mediated RNA trans-splicingNature Medicine, 2003
- Optical imaging of transferrin targeted PEI/DNA complexes in living subjectsGene Therapy, 2003
- Antisense oligonucleotide therapy in cancer.2003
- Optical bioluminescence and positron emission tomography imaging of a novel fusion reporter gene in tumor xenografts of living mice.2003
- Monitoring Protein−Protein Interactions Using Split Synthetic Renilla Luciferase Protein-Fragment-Assisted ComplementationAnalytical Chemistry, 2003
- Molecular imaging in living subjects: seeing fundamental biological processes in a new lightGenes & Development, 2003
- Optical imaging of Renilla luciferase reporter gene expression in living miceProceedings of the National Academy of Sciences, 2001
- Messenger RNA Repair and Restoration of Protein Function by Spliceosome-Mediated RNA Trans-SplicingMolecular Therapy, 2001
- Changes in size and secondary structure of the ribosomal transcription unit during vertebrate evolutionJournal of Molecular Biology, 1975