Comparison of Different Immunogen Preparations for Serological Identification ofXanthomonas campestrispv.campestris

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Abstract
Four immunogens; formaldehyde fixed cells, glutaraldehdye fixed cells, trichloroacetic acid extracts and ribosomal extracts, were compared for identification of X. campestris pathovar campestris (XC). In Ouchterlony double diffusion (ODD) tests, antiserum (AS) to each of the immunogens resulted in a single major band and 1-3 minor bands of precipitin when reacted against homologous cell extracts. A reaction of complete fusion (identity) occurred between the major band of precipitin of the 4 immunogens. The major precipitin was considerably sharper and stronger in the AS to ribosomes. By means of AS to ribosomes and the major precipitin, 25 strains of XC were typed into 4 serovars. Other bacteria (32), including 16 strains of 5 other pathovars of XC, 12 strains of 5 other genera and 4 unidentified bacteria from crucifer seeds, were tested by ODD with AS to the 4 serovars. Three strains of X. vesicatoria and 1 strain of X. translucens cross-reacted; all other bacteria failed to react. Immunogens (2-4) were identified in immunoelectrophoresis. The major band of precipitin was identified as a neutral immunogen. In immunofluorescence (IF) tests, few differences were observed among the 4 immunogens. The greatest specificity was found with AS to glutaraldehyde and formaldehyde fixed cells. None of the immunogens was specific enough in IF to differentiate XC from other xanthomonads.

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