A requirement for EDTA in the separation of Photosystems 1 and 2 from the cyanobacterium Chlorogloea fritschii
- 15 May 1981
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 196 (2) , 489-493
- https://doi.org/10.1042/bj1960489
Abstract
Fractions enriched in Photosystem 1 or Photosystem 2 activity have been isolated from the cyanobacterium Chlorogloea fritschii after extraction of the membranes with digitonin and Triton X-100. Separation of the extract into the two components was achieved by using a Sepharose 6B column, calibration of which gave Kd values of 0.3 for the Photosystem 1 fraction and 0.53 for Photosystem 2. These values corresponded to molecular weights of approx. 500000 and 90000 respectively. The Photosystem 1 particle was shown to aggregate on storage and EDTA was shown to be necessary to separate the Photosystem 1 and 2 fractions.This publication has 13 references indexed in Scilit:
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