Studies on the Formation of Aminopeptidase-like Enzymes in Regenerating Wound Tissue.

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Abstract
The activity of aminopeptidase-like enzymes from the regenerating wound tissue of the rat was studied. The enzyme activity hydrolysing 2-naphthyl-amides of L-leucine and DL-alanine began to increase about 2 hr. after the injury, whereas that affecting 2-naphthylamide and p-nitro-anilide of L-lysine was increased as soon as 1 hr. after the injury. The wound enzymes were fractionated by ammonium sulphate precipi-tation and by CM-cellulose chromatography. The stability of the enzymes involved were studied. Different, wound specific amino-peptidase-like enzymes exist.
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