Radioactive sugars were introduced in soybean plants either through the stump of a cut petiole or through a flap formed by a cut lateral vein of a leaf. After 5 to 180 minutes the location, distribution, and nature of the tracer was determined. When sucrose, glucose, and mannitol were introduced via a cut petiole, they were translocated in the stem via the xylem and they passed through a steam girdle contrary to results previously reported in which sucrose transport appeared to be blocked. When labelled glucose was introduced via a lateral leaf vein it was metabolized and translocated as sucrose from the leaf via the phloem in a manner closely resembling that of photosynthate.