MEASUREMENT OF THE RATE OF UPTAKE AND SUBCELLULAR LOCALIZATION OF PORPHYRINS IN CELLS USING FLUORESCENCE DIGITAL IMAGING MICROSCOPY
- 1 April 1994
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 59 (4) , 419-422
- https://doi.org/10.1111/j.1751-1097.1994.tb05058.x
Abstract
A fluorescence imaging system incorporating a cooled slow‐scan charge‐coupled device camera was used to study the rate of uptake and subcellular localization of porphyrins in living cells. Measurements were carried out on human dermal fibroblasts (D532) using two different porphyrins meso‐tetra(4‐N‐methylpyridyl)porphine (TMPP) and meso‐tetra(4‐N‐hexylpyridyl)porphine (THPP). It was observed that TMPP was rapidly taken up by cells and principally located in the nucleus. The THPP, on the other hand, internalized more slowly and exhibited a particulate distribution in the cytoplasm.Keywords
This publication has 10 references indexed in Scilit:
- Intracellular Localization of PhotosensitizersPublished by Wiley ,2007
- SUBCELLULAR LOCALIZATION OF PORPHYRINS USING CONFOCAL LASER SCANNING MICROSCOPYPhotochemistry and Photobiology, 1991
- Ultrastructural changes in the nuclei of human carcinoma cells after photodynamic treatment with haematoporphyrin derivative and tetrasodium-meso-tetra-(4-sulphonatophenyl)porphineLasers in Medical Science, 1988
- The Use of a Charge-Coupled Device for Quantitative Optical Microscopy of Biological StructuresScience, 1987
- PHOTOSENSITIZERS: THERAPY AND DETECTION OF MALIGNANT TUMORSPhotochemistry and Photobiology, 1987
- Distribution of endogenous and injected porphyrins at the subcellular level in rat hepatocytes and in ascites hepatomaChemico-Biological Interactions, 1981
- Interaction of DNA with a porphyrin ligand: evidence for intercalationNucleic Acids Research, 1979
- Synthesis and characterization of new isomeric water-soluble porphyrins. Tetra(2-N-methylpyridyl)porphine and tetra(3-N-methylpyridyl)porphineInorganic Chemistry, 1976
- Aggregation of meso-substituted water-soluble porphyrinsJournal of the American Chemical Society, 1972
- α,β,γ,δ-Tetra-(4-pyridyl)-porphine and Some of its Metal ComplexesInorganic Chemistry, 1962