A Penicillium freii gene that is highly similar to the β‐keto‐acyl synthase domain of polyketide synthase genes from other fungi
Open Access
- 1 September 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 25 (3) , 197-201
- https://doi.org/10.1046/j.1472-765x.1997.00005.x
Abstract
A Penicillium freii DNA fragment with similarity to the β-keto-acyl synthase motif of the P. griseofulvum 6-methylsalicylic acid synthase encoding gene (MSAS) was identified by screening a cosmid library using a part of MSAS as the probe. Two exons of 93 and 849 bp, encoding a predicted polypeptide of 314 amino acids, and a molecular mass of 33·4 kDa, were identified (PfKS). PfKS was transcribed as a 1·6 kbp messenger. A region corresponding to the MSAS gene encoding essential enzyme activities for the assembly of a polyketide chain was absent in PfKS. Gene disruption experiments in P. freii with a truncated version of PfKS did not result in the detection of homologous integration events.Keywords
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