Mouse Hybrid Cell Lines produce Antibodies to Herpes Simplex Virus Type 1

Publisher Website
Google Scholar
Abstract
A solid-phase radioimmunoassay procedure was devised for the assay of antibodies produced in the mouse to herpes simplex virus type 1 (HSV-1). It is based on the adsorption of virus to flexible micro-well plates and uses radio-iodine-labeled rabbit antibody against mouse immunoglobulin to assess antibody binding. Using this assay for screening, cell hybrids were obtained which yield monoclonal antibody to HSV-1. The hybrids are between spleen cells from hyperimmune mice and an immunoglobulin-non-secreting, azaguanine resistant myeloma cell line (NS-1). From 480 hybrid cell lines initially examined, 5 stable cell lines were obtained which released HSV-1-specific antibody in vitro and in vivo. Mice carrying transplants of these cell lines yield binding titers in serum of up to 1/25,000. Ig[immunoglobulin]G and IgM antibodies were obtained in this way.

This publication has 1 reference indexed in Scilit: