THE EFFECT OF BIOTIN ON THE METABOLISM OF LIVER SLICES FROM BIOTINDEFICIENT RATS

Abstract
Rats were made biotin-deficient by a diet containing 30-40% egg white and no "butter-yellow." Small amts. of biotin (as little as 2 parts in 10 million of medium) added to slices of biotin-deficient rat liver respiring in Ringer-bicarbonate soln. containing lactate or pyruvate, produced a significant effect on the liver metabolism. The chemical data and the differential manometer of Summerson showed a slight rise in O2 consumption and R. Q. when biotin was added, and a very marked change in the aerobic glycolysis in a negative direction because of increased bicarbonate production.

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