Studies on Electrophoresis on Cellulose Acetate Membrane of Bovine Serum Proteins in Healthy Animals

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Abstract
A method for rapid electrophoresis of serum proteins on a cellulose acetate membrane is described. In order to experimentally identify the various larger globulin fractions in normal bovine serum, pure bovine y-globulin and a bovine attraction (Antitrombin III) were used. These fractions were electrophoretically compared with bovine serum and the various fractions on the diagram determined. A diluted veronal buffer with added Ga-acetate gave considerably better separation than the reported standard buffer. In the last part of the experiment normal percentage values for the composition of the protein fractions in bovine serum were calculated by the method described. The material consisted of 50 cows of the N.R.F. breed. The values are compared with corresponding figures reported by other authors, and the results are discussed.