Measurement of Binding of Chloramphenicol by Intact Cells
- 1 May 1967
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 93 (5) , 1671-+
- https://doi.org/10.1128/jb.93.5.1671-1676.1967
Abstract
The binding of chloramphenicol to intracellular components of intact cells was measured by procedures based on a silicone-wash technique. The number of stereospecifically bound molecules of chloramphenicol increased with external concentration to a saturation value equal to the number of ribosomes per cell. Chloramphenicol is therefore believed to be attached stereospecifically by a weak bond, most probably to a single site on the 50 S ribosome. This bond was found to be temperature-dependent and appeared to be responsible for inhibition of protein synthesis.This publication has 8 references indexed in Scilit:
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