Quantitative Detection of Hepatitis C Virus RNA by Light Cycler PCR and Comparison with Two Different PCR Assays
Open Access
- 1 February 2001
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 39 (2) , 765-768
- https://doi.org/10.1128/jcm.39.2.765-768.2001
Abstract
The new Light Cycler technology was adapted to the detection of hepatitis C virus (HCV) RNA in clinical samples. Sera from 81 patients were tested by Light Cycler PCR, AMPLICOR HCV Monitor assay, and in-house PCR. Our data demonstrate that Light Cycler is a fast and reliable method for the detection and quantitation of HCV RNA.Keywords
This publication has 24 references indexed in Scilit:
- Different Real-Time PCR Formats Compared for the Quantitative Detection of Human Cytomegalovirus DNAClinical Chemistry, 1999
- Use of Real-Time PCR and Fluorimetry To Detect Lamivudine Resistance-Associated Mutations in Hepatitis B VirusAntimicrobial Agents and Chemotherapy, 1999
- Feasibility and efficacy of routine PCR screening of blood donations for hepatitis C virus, hepatitis B virus, and HIV-1 in a blood-bank settingThe Lancet, 1999
- Real time quantitative PCR.Genome Research, 1996
- Pretreatment serum hepatitis C virus RNA levels and hepatitis C virus genotype are the main and independent prognostic factors of sustained response to interferon alfa therapy in chronic hepatitis CHepatology, 1995
- Quantitative PCR: Theoretical Considerations with Practical ImplicationsAnalytical Biochemistry, 1993
- Antibody response to core, envelope and nonstructural hepatitis C virus antigens: Comparison of immunocompetent and immunosuppressed patientsHepatology, 1993
- Quantitative analysis of hepatitis C virus RNA in serum during interferon alfa therapyGastroenterology, 1993
- Transmission of Hepatitis C Virus by Organ TransplantationNew England Journal of Medicine, 1991
- Isolation of a cDNA cLone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis GenomeScience, 1989