The Na+‐independent Ca2+ efflux system in mitochondria is a Ca2+/2H+ exchange system
- 12 November 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 274 (1-2) , 65-68
- https://doi.org/10.1016/0014-5793(90)81330-q
Abstract
The mechanism of the Na + -independent Ca 2+ efflux system in mitochondria has not been elucidated as yet. With the aid of cyclosporin A. an inhibitor of the Ca 2+ -induced ‘pore’, and using a variety of inhibitors, uncouplers and lonophores, it is possible to demonstrate, unequivocally, that this process is driven by ΔpH. The efflux is not affected by ΔΨ, thus suggesting an electroneutral Ca 2+ /2H + exchange mechanism. Parallel measurements of the rate of Ca 2+ efflux and ΔpH, as modulated by valinomycin and nigericin, indicate that the rate of efflux is a function of the magnitude of ΔpH.Keywords
This publication has 17 references indexed in Scilit:
- Regulation of Ca2+ transport in brain mitochondria. I. The mechanism of spermine enhancement of Ca2+ uptake and retentionBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
- Regulation of Ca2+ transport in brain mitochondria. II. The mechanism of the adenine nucleotides enhancement of Ca2+ uptake and retentionBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
- Surface potential in rat liver mitochondria: terbium ion as a phosphorescent probe for surface potentialBiochemistry, 1983
- Mechanism of sodium ion independent calcium ion efflux from rat liver mitochondriaBiochemistry, 1983
- The Role of Phosphate in the Regulation of the Independent Calcium‐Efflux Pathway of Liver MitochondriaEuropean Journal of Biochemistry, 1982
- Ruthenium red sensitive and insensitive calcium transport in rat liver and Ehrlich ascites tumor cell mitochondriaFEBS Letters, 1978
- Effect of pH and Ca2+ on the retention of Ca2+ by rat liver mitochondriaArchives of Biochemistry and Biophysics, 1978
- Relation between the Gradient of the ATP/ADP Ratio and the Membrane Potential across the Mitochondrial MembraneEuropean Journal of Biochemistry, 1977
- Calcium uptake and membrane potential in mitochondriaBiochemistry, 1974
- Respiratory Control and the Proton Electrochemical Gradient in MitochondriaEuropean Journal of Biochemistry, 1973