In vitro reconstitution of hnRNP particles
- 10 April 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 247 (1) , 51-54
- https://doi.org/10.1016/0014-5793(89)81238-4
Abstract
The assembly of hnRNP‐like particles was studied by in vitro reconstitution, UV‐crosslinking and CsCl‐equilibrium centrifugation. Using total nuclear protein and RNA extracts from HeLa cells for RNP reconstitution, RNP particles sedimenting with the same buoyant density of ϱ=1.4 g/cm3 as ‘native’ 40 S core hnRNPs were obtained. Under the stringent reconstitution conditions used, hnRNP complexes containing only the C1‐core hnRNP protein could be identified.Keywords
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