Production and Characterization of Monoclonal Antibodies to Rat Plasma Kininogen
- 1 October 1987
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 6 (5) , 521-526
- https://doi.org/10.1089/hyb.1987.6.521
Abstract
Five hybridoma cell lines were established that secrete monoclonal antibodies (MAbs) directed against rat plasma low molecular weight plasma kininogen. Two of the secreted antibodies were of the IgG1k isotype. The remaining three were of the IgG1λ, IgG2ak and IgMk isotypes, respectively. The dissociation constants (Kd) of these MAbs ranged from 0.58x10-9M to 5.4x10-9M. At least two distinct epitopes on the rat plasma kininogen were recognized by these MAbs. Further characterization of the MAbs showed that four MAbs cross-reacted strongly with kininogen from the Murphy-Sturm lymphosarcoma (MSLS) tumor while one of the MAbs cross-reacted weakly with MSLS tumor kininogen.This publication has 7 references indexed in Scilit:
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