Effect of single‐point mutations Phe41→ His and Phe143→ Glu on folding and catalytic properties of recombinant horseradish peroxidase expressed in E. coli
- 14 November 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 354 (3) , 248-250
- https://doi.org/10.1016/0014-5793(94)01125-7
Abstract
Wild-type recombinant and Phe41→ His and Phe143→ Glu mutant forms of horseradish peroxidase have been expressed in E. coli and reactivated from inclusion bodies with a yield of about 25%. The purified homogeneous preparations have been studied in the reaction of ABTS oxidation. The effect of mutations on heme entrapment and kinetics of ABTS oxidation demonstrates the essential role of the replaced residues in providing the hydrophobic crevice for the non-covalent heme binding.Keywords
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