AMINO NITROGEN OF SOILS AND OF CERTAIN FRACTIONS ISOLATED FROM THEM

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Abstract
A preliminary study of the reaction of 1-fluoro-2, 4-dinitroben-zene with soil materials indicated that there were no free amino groups in the soils studied or in organic fractions isolated from them. A small amt. of amino N was set free from soil fractions by boiling 1% HC1, 5% NaHCO3 at 50[degree]C , and concentrated CH3.COOH-HCl at 37[degree]C. A rather complex mixture of dinitrophenyl amino acids was formed, and there was no clear evidence that any specific peptide linkage was broken preferentially, although with the CH3.COOH-HCl hydrolysis, dinitrophenyl derivatives of the hydroxyamino acids were often found. The indications were that a complex mixture of peptides was formed by partial hydrolysis.