Measurement of cytoplasmic pH of the alkaliphile Bacillus lentus C-125 with a fluorescent pH probe
- 1 August 1997
- journal article
- Published by Microbiology Society in Microbiology
- Vol. 143 (8) , 2531-2536
- https://doi.org/10.1099/00221287-143-8-2531
Abstract
Summary: A method was established to measure the cytoplasmic pH of the facultative alkaliphilic strain, Bacillus lentus C-125. The bacterium was loaded with a pH-sensitive fluorescent probe, 2′,7′-bis-(2-carboxyethyl)-5 (and -6)-carboxyfluorescein (BCECF), and cytoplasmic pH was determined from the intensity of fluorescence of the intracellular BCECF. The activity of the organism to maintain neutral cytoplasmic pH was assessed by measuring the cytoplasmic pH of the cells exposed to various pH conditions. The cytoplasmic pH maintenance activity of C-125 increased with increasing culture pH, indicating that the activity was regulated in response to the culture pH.Keywords
This publication has 16 references indexed in Scilit:
- Alkaline Growth pH-Dependent Increase of Respiratory and NADH-Oxidation Activities of the Facultatively Alkaliphilic StrainBacillus lentusC-125Bioscience, Biotechnology, and Biochemistry, 1996
- A high cell wall negative charge is necessary for the growth of the alkaliphile Bacillus lentus C-125 at elevated pHMicrobiology, 1995
- Assignment of Facultatively Alkaliphilic Bacillus sp. Strain C-125 to Bacillus lentus Group 3International Journal of Systematic and Evolutionary Microbiology, 1995
- Alkaliphiles:‘basic’molecular problems of pH tolerance and bioenergeticsMolecular Microbiology, 1995
- Properties of two different Na+/H+ antiport systems in alkaliphilic Bacillus sp. strain C-125Journal of Bacteriology, 1994
- Characteristics of Alkali-sensitive Mutants of AlkaliphilicBacillussp. Strain C-125 That Show Cellular Morphological AbnormalitiesBioscience, Biotechnology, and Biochemistry, 1994
- Hyper-autolysis of the Facultative AlkaliphileBacillussp. C-125 Cells Grown at Neutral pH: Culture-pH Dependent Cross-linking of the Peptide Moieties of the PeptidoglycanBioscience, Biotechnology, and Biochemistry, 1994
- Continuous measurement of the cytoplasmic pH in Lactococcus lactis with a fluorescent pH indicatorBiochimica et Biophysica Acta (BBA) - General Subjects, 1991
- The sodium/proton antiport system in a newly isolated alkalophilic Bacillus spJournal of Bacteriology, 1989
- [56] Determination of the net proton-hydroxide ion permeability across vesicular lipid bilayers and membrane proteins by optical probesPublished by Elsevier ,1986