Role of the leader sequence in tobacco pectin methylesterase secretion

8 May 2006

journal article
Published by Wiley in FEBS Letters

Vol. 580 (13) , 3329-3334
https://doi.org/10.1016/j.febslet.2006.04.090

Abstract

We report that unprocessed tobacco pectin methylesterase (PME) contains N-terminal pro-sequence including the transmembrane (TM) domain and spacer segment preceding the mature PME. The mature portion of PME was replaced by green fluorescent protein (GFP) gene and various deletion mutants of pro-sequence fused to GFP were cloned into binary vectors and agroinjected in Nicotiana benthamiana leaves. The PME pro-sequence delivered GFP to the cell wall (CW). We showed that a transient binding of PME TM domain to endoplasmic reticulum membranes occurs upon its transport to CW. The CW targeting was abolished by various deletions in the TM domain, i.e., anchor domain was essential for secretion of GFP to CW. By contrast, even entire deletion of the spacer segment had no influence on GFP targeting.

Keywords

This publication has 26 references indexed in Scilit:

Pectin Methylesterases and Pectin Dynamics in Pollen Tubes
Plant Cell, 2005
Sec22 and Memb11 Are v-SNAREs of the Anterograde Endoplasmic Reticulum-Golgi Pathway in Tobacco Leaf Epidermal Cells
Plant Physiology, 2005
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant Cells[W]
Plant Cell, 2004
Membrane Protein Transport between the Endoplasmic Reticulum and the Golgi in Tobacco Leaves Is Energy Dependent but Cytoskeleton Independent
Plant Cell, 2002
Redistribution of membrane proteins between the Golgi apparatus and endoplasmic reticulum in plants is reversible and not dependent on cytoskeletal networks
The Plant Journal, 2002
A novel function for a ubiquitous plant enzyme pectin methylesterase: the host‐cell receptor for the tobacco mosaic virus movement protein
FEBS Letters, 1999
Effect of Pectin Methylesterase Gene Expression on Pea Root Development
Plant Cell, 1999
Heads or tails — what determines the orientation of proteins in the membrane
FEBS Letters, 1995
An Antisense Pectin Methylesterase Gene Alters Pectin Chemistry and Soluble Solids in Tomato Fruit.
Plant Cell, 1992
An Antisense Pectin Methylesterase Gene Alters Pectin Chemistry and Soluble Solids in Tomato Fruit
Plant Cell, 1992