pED100, a conjugative F plasmid derivative without insertion sequences
- 1 August 1981
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 182 (3) , 520-522
- https://doi.org/10.1007/bf00293948
Abstract
The largest HindIII fragment of F includes the entire replication and transfer regions, and its circularisation with ligase gave the conjugative plasmid pED100. This plasmid, which contains none of the F insertion sequences, was essentially unable to mobilise the E. coli chromosome or to give integrative suppression of a dnaA strain.Keywords
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