Analysis of Early Human Immunodeficiency Virus Type 1 DNA Synthesis by Use of a New Sensitive Assay for Quantifying Integrated Provirus
Open Access
- 15 September 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (18) , 10119-10124
- https://doi.org/10.1128/jvi.77.18.10119-10124.2003
Abstract
A novel Alu -long terminal repeat (LTR)-based real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type 1 (HIV-1) DNA in infected cells with both accuracy and high sensitivity (six proviruses within 50,000 cell equivalents). Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication.Keywords
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