Peptones and Calf Serum as a Replacement for Human Serum in the Cultivation of Plasmodium falciparum

Publisher Website
Google Scholar
Abstract
Attempts were made to find an inexpensive, readily available substitute for the human serum requirement for the continuous culture of erythrocytic stages of P. falciparum. Neopeptone or Proteose-Peptone No. 3 added together with calf serum gave parasite growth rates comparable to, or surpassing, those obtained with human serum. It was first necessary to adapt the parasites by a gradual, stepwise reduction in the amount of human serum used, and a concomitant, stepwise increase in the substitutes added.