Lymphocyte stimulation: a rapid multiparameter analysis.

1 August 1976

journal article
research article
Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences

Vol. 73 (8) , 2881-2884
https://doi.org/10.1073/pnas.73.8.2881

Abstract

Several parameters of stimulation of individual lymphocytes [human] were measured simultaneously by flow-cytofluorometry after differential staining of cellular DNA and RNA with the metachromatic fluorescent dye acridine orange. The method provides a means of analyzing the progression of stimulated cells through the cell cycle (G1, S and G2 + M), in addition to measuring the accumulation of RNA per cell; the RNA parameter is useful in distinguishing G1 from G0 cells. The multiparameter analysis may be of unique value in discriminating cases where the transcriptional and proliferative responses of lymphocytes to stimulants are not correlated.

Keywords

This publication has 18 references indexed in Scilit:

Conformation of RNA in situ as studied by acridine orange staining and automated cytofluorometry
Experimental Cell Research, 1975
Automated cytology.
1975
Suppression of RNA synthesis in lymphocytes by inhibitors of proteolytic enzymes
Experimental Cell Research, 1974
DISCUSSION PAPER: STUDIES ON THE DIFFERENTIATION WITHIN THE IMMUNE SYSTEM
Annals of the New York Academy of Sciences, 1973
Selective Stimulation of IgM Synthesis in Mouse B Lymphocytes by Pokeweed Mitogen
Nature New Biology, 1972
Effects of inhibition of ribosomal RNA synthesis on the stimulation of lymphocytes by phytohaemagglutinin
Experimental Cell Research, 1969
Microfluorometric characterization of intracellular nucleic acids and nucleoproteins by acridine orange.
1966
THE STRUCTURE OF THE DNA-ACRIDINE COMPLEX
Proceedings of the National Academy of Sciences, 1963
PHYTOHEMAGGLUTININ - AN INITIATOR OF MITOSIS IN CULTURES OF NORMAL HUMAN LEUKOCYTES
1960
AGGREGATION OF DYES BOUND TO POLYANIONS
Proceedings of the National Academy of Sciences, 1959