Antigen presentation in the murine T-lymphocyte proliferative response. I. Requirement for genetic identity at the major histocompatibility complex
Open Access
- 1 September 1977
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 146 (3) , 828-843
- https://doi.org/10.1084/jem.146.3.828
Abstract
A method is described for stimulating proliferation in primed populations of murine T [thymus-derived] lymphocytes using antigen bound to mitomycin-C-treated spleen cells. This form of antigen presentation appears to be an active process because heat-killed spleen cells are ineffective, and because genetic similarity at the major histocompatibility complex (MHC) between the responder T cells and the presenting spleen cells is required for effective interactions. At all times examined, from day 3 to day 6 of the proliferative response, syngeneic spleen cells presented antigen better to peritoneal exudate T-lymphocyte-enriched cells (PETLES) than semisyngeneic F1 spleen cells, which in turn could present antigen better than totally allogeneic spleen cells. Spleen cell mixing experiments demonstrated that these genetic restrictions were not the result of suppression by the ongoing mixed lymphocyte reactions (MLR) in the allogeneic and F1 cases. Incompatibility at the Mls locus generated a strong MLR but failed to prevent antigen presentation if the spleen cells and PETLES were H-2 compatible. Genetic mapping studies demonstrated that compatibility at only the I-A subregion of the MHC was sufficient for effective presentation of the antigen, dinitrophenylated ovalbumin. Compatibility at only the K region, or the K and D regions was not sufficient. Functional activation of primed, proliferating T lymphocytes requires the participation of gene products coded for by the I region of the MHC. This conclusion is consistent with a growing body of evidence which suggests that most T cells recognize antigen in association with MHC gene products.This publication has 26 references indexed in Scilit:
- Functional Specificity of Thymus-Dependent LymphocytesScience, 1977
- Helper T cells recognize antigen and macrophage surface components simultaneouslyNature, 1976
- Participation of the H-2 antigens of tumor cells in their lysis by syngeneic T cells.The Journal of Experimental Medicine, 1976
- T-lymphocyte-enriched murine peritoneal exudate cells. II. Genetic control of antigen-induced T-lymphocyte proliferation.The Journal of Experimental Medicine, 1976
- H-2 gene complex restricts transfer of delayed-type hypersensitivity in mice.Proceedings of the National Academy of Sciences, 1975
- The role of macrophages in the generation of T-helper cells. II. The genetic control of the macrophage-T-cell interaction for helper cell induction with soluble antigens.The Journal of Experimental Medicine, 1975
- Cell interactions between histoincompatible T and B lymphocytes. VII. Cooperative responses between lymphocytes are controlled by genes in the I region of the H-2 complexThe Journal of Experimental Medicine, 1975
- FUNCTION OF MACROPHAGES IN ANTIGEN RECOGNITION BY GUINEA PIG T LYMPHOCYTESThe Journal of Experimental Medicine, 1973
- CELL INTERACTIONS BETWEEN HISTOINCOMPATIBLE T AND B LYMPHOCYTESThe Journal of Experimental Medicine, 1973
- CRITICAL ROLE OF DETERMINANT PRESENTATION IN THE INDUCTION OF SPECIFIC RESPONSES IN IMMUNO-COMPETENT LYMPHOCYTESThe Journal of Experimental Medicine, 1973