Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase
- 1 December 2003
- journal article
- Published by Bentham Science Publishers Ltd. in Protein & Peptide Letters
- Vol. 10 (6) , 575-580
- https://doi.org/10.2174/0929866033478546
Abstract
Previously we reported that halobacterial nucleoside diphosphate kinase can be refolded in the presence of concentrated trimethylamine N-oxide (TMAO) as well as NaCl, indicating that enhancement of compact structure formation by TMAO is sufficient for folding. Here we showed that the refolding effect of MgCl2 is maximal at 1 M and declines to zero at 2 M, indicating that charge shielding effect of MgCl2 is offset by its salting-in effect.Keywords
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