Light-dependent in vivo phosphorylation of an inhibitory subunit of cGMP-phosphodiesterase in frog rod photoreceptor outer segments
- 31 January 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 338 (2) , 203-206
- https://doi.org/10.1016/0014-5793(94)80365-x
Abstract
In vivo phosphorylation of Pγ, an inhibitory subunit of cGMP-phosphodiesterase of frog (Rana catesbeiana) photoreceptor rod outer segments, was investigated using a quick-freezing technique and a newly developed method for the preparation of rod outer segments. Light-dependent phosphorylation of Pγ was observed. Okadaic acid, a potent inhibitor of protein phosphatases 1 and 2A, enhanced the apparent incorporation of 32P into Pγ suggesting that Pγ is in equilibrium between phosphorylation and dephosphorylation. Neither phorbol ester, a potent activator of protein kinase C, nor changes in the extracellular Ca2+ concentration affected the in vivo phosphorylation of PγKeywords
This publication has 11 references indexed in Scilit:
- Regulation of deactivation of photoreceptor G protein by its target enzyme and cGMPNature, 1992
- Phosphatidylinositol-stimulated phosphorylation of an inhibitory subunit of cGMP phosphodiesterase in vertebrate rod photoreceptors.Proceedings of the National Academy of Sciences, 1991
- G protein-effector coupling: binding of rod phosphodiesterase inhibitory subunit to transducinBiochemistry, 1989
- cGMP phosphodiesterase of retinal rods is regulated by two inhibitory subunits.Proceedings of the National Academy of Sciences, 1988
- Activation of retinal rod cyclic GMP‐phosphodiesterase by transducin: Characterization of the complex formed by phosphodiesterase inhibitor and transducin α‐subunitProteins-Structure Function and Bioinformatics, 1986
- Two-Dimensional Gel Electrophoresis: A Guide for the BeginnerPublished by Elsevier ,1984
- Light-Induced Modification of Drosophila Retinal Polypeptides in VivoScience, 1982
- Flow of information in the light-triggered cyclic nucleotide cascade of vision.Proceedings of the National Academy of Sciences, 1981
- A light-activated GTPase in vertebrate photoreceptors: Regulation of light-activated cyclic GMP phosphodiesteraseProceedings of the National Academy of Sciences, 1977
- Assay of proteins in the presence of interfering materialsAnalytical Biochemistry, 1976