Enzyme-linked immunosorbent assay for detection of capsular antibodies against Haemophilus influenzae type b: comparison with radioimmunoassay
- 1 November 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 18 (5) , 1201-1204
- https://doi.org/10.1128/jcm.18.5.1201-1204.1983
Abstract
An enzyme-linked immunosorbent assay (ELISA) is described for detection of anti-capsular antibodies against H. influenzae type b. Polyribosephosphate was covalently bonded to poly-L-lysine before adsorption to microtiter plates. ELISA IgG and IgM anti-polyribosephosphate antibody titers were comparable to total anti-polyribosephosphate antibody concentration determined by radioimmunoassay. The ELISA technique will be useful for further investigations of host response to infections due to H. influenzae type b, but is not intended to be used as a serological method for documenting H. influenzae type b infections.This publication has 13 references indexed in Scilit:
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