Identification of Acetylcholine in Sympathetic Ganglia by Chemical and Physical Methods

Abstract
Extracts of sympathetic ganglionic chains contain a substance which behaves like acetylcholine biologically, chromatographically, and electrophoretically. The melting point of the tetrachloroaurate salt of this substance is identical to that of acetylcholine tetrachloroaurate. No other choline esters have been detected in these extracts. Perfusion of sympathetic ganglia with C14-choline indicates that C14-acetylcholine is released.

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