THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS IN VITRO, I. DEDIFFERENTIATION OF CARTILAGE CELLS
Open Access
- 1 December 1960
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 46 (12) , 1533-1542
- https://doi.org/10.1073/pnas.46.12.1533
Abstract
Chondrocytes liberated from embryonic cartilage by means of trypsin and immediately organ-cultured continue to synthesize chondrotin sulfate and incorporate inorganic S35 into ester-bound sulfate. After being cultured under conditions that suppress differentiation but encourage cell multiplication, these cells, when organ-cultured, (1) fail to differentiate into recognizable chondrocytes, (2) fail to synthesize chondroitin sulfate, and (3) fail to incorporate S35 into ester-bound sulfate. Dedifferentiated cartilage cells may differentiate into fat cells.Keywords
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