Purification of African Trypanosomes Can Cause Biochemical Changes in the Parasites1
- 1 November 1987
- journal article
- research article
- Published by Wiley in The Journal of Protozoology
- Vol. 34 (4) , 405-408
- https://doi.org/10.1111/j.1550-7408.1987.tb03201.x
Abstract
Bloodstream forms of African trypanosomes are routinely purified from blood components by a combination of centrifugation and chromatography on DEAE cellulose at pH 8.0. Here we report that the nonphysiological conditions used for DEAE chromatography of the parasites result in changes in the ATP levels of the trypanosomes and an enhanced release from the parasites of proteins such as variable surface glycoprotein, peptidase, and phospholipase. Some of these changes can be reduced by the addition of nucleosides to the elution buffer and, after the elution of the parasites, by immediate readjustment of the external pH to the normal physiological level of blood (pH 7.4).This publication has 16 references indexed in Scilit:
- Thiol‐dependent proteases of African trypanosomesEuropean Journal of Biochemistry, 1986
- Glycosyl-sn-1,2-dimyristylphosphatidylinositol is covalently linked to Trypanosoma brucei variant surface glycoprotein.Journal of Biological Chemistry, 1985
- A new method for the rapid purification of both the membrane-bound and released forms of the variant surface glycoprotein from Trypanosoma bruceiBiochemical Journal, 1985
- The intracellular pathway and assembly of newly formed variable surface glycoprotein of Trypanosoma brucei.Proceedings of the National Academy of Sciences, 1984
- Purification and Properties of the Membrane Form of Variant Surface Glycoproteins (VSGs) from Trypanosoma brucei1,2,3The Journal of Protozoology, 1984
- A micromethod for the quantitation of cellular proteins in Percoll with the Coomassie brilliant blue dye-binding assayAnalytical Biochemistry, 1983
- Purine metabolism in the bloodstream forms of trypanosoma gambiense and Trypanosoma rhodesienseBiochimica et Biophysica Acta (BBA) - General Subjects, 1982
- The phospholipases of Trypanosoma brucei bloodstream forms and cultured procyclicsMolecular and Biochemical Parasitology, 1982
- Crossreacting determinants in variant-specific surface antigens of African trypanosomes.Proceedings of the National Academy of Sciences, 1978
- Isolation of salivarian trypanosomes from man and other mammals using DEAE-celluloseExperimental Parasitology, 1970