Uptake of Radioactive D-Glucose Anomers by Pancreatic Islets

Abstract
Isolated rat islets were incubated in media containing either the α or β anomer of D-[1–3H]glucose for 5 min at 37°. The amounts of the two anomers incorporated were determined using L-[1–14C]glucose as an extracellular space marker. The incorporation of β-D-glucose was about twice that of α-D-glucose. Our previous and present results suggest that the two anomers of D-glucose each have a preferential function in pancreatic beta cells; α-D-glucose stimulates insulin secretion, and β-D-glucose is transported into the cells.

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