A polyclonal antiserum against the rabbit progesterone receptor recognizes the human receptor: Immunohistochemical localization in rabbit and human uterus
- 1 April 1989
- journal article
- research article
- Published by Wiley in Cell Biochemistry and Function
- Vol. 7 (2) , 147-152
- https://doi.org/10.1002/cbf.290070211
Abstract
A polyclonal antiserum, raised in guinea pigs immunized with the 116 000 Mr rabbit uterine progesterone receptor (PR), was used to demonstrate immunoreactive PR in frozen fixed sections of rabbit and human uterus. In both species, PR localization was exclusively nuclear. For the rabbit uterus, staining intensity was greatest in the myometrium, followed by endometrial stroma, glands, and luminal epithelium. In premenopausal human endometrium and myometrium there was intense staining of nuclei from proliferative phase glands and myometrium. In the secretory phase the glands failed to stain, yet immunostaining persisted in the myometrium.Keywords
This publication has 13 references indexed in Scilit:
- A polyclonal antiserum against the rabbit progesterone receptor recognizes the human receptor: Biochemical characterizationCell Biochemistry and Function, 1989
- Monoclonal Antibodies to Human Progesterone Receptor: Characterization by Biochemical and Immunohistochemical Techniques*Endocrinology, 1987
- Effect of photoaffinity labeling on rabbit uterine progesterone receptorAnalytical Biochemistry, 1986
- The Effect of Progestins on the Mitotic Activity of Uterine FibromyomasInternational Journal of Gynecological Pathology, 1985
- Immunocytochemical Study of Mammalian Progesterone Receptor Using Monoclonal Antibodies*Endocrinology, 1985
- Monoclonal antibodies localize oestrogen receptor in the nuclei of target cellsNature, 1984
- Heterogeneity and progesterone-receptor distribution in endometrial adenocarcinomaCancer, 1984
- Distribution of Progesterone Receptor, Estradiol Dehydrogenase, and 20α-Dihydroprogesterone Dehydrogenase Activities in Human Endometrial Glands and Stroma: Progestin Induction of Steroid Dehydrogenase Activities in Vitro Is Restricted to the Glandular Epithelium*Endocrinology, 1982
- Leiomyomas: Steroid receptor contentAmerican Journal of Obstetrics and Gynecology, 1982
- Determination of Estradiol, Estrone, and Progesterone in Serum and Myometrium: Correlation with the Content of Sex Steroid Receptors and 17β-Hydroxysteroid Dehydrogenase Activity throughout the Menstrual CycleJournal of Clinical Endocrinology & Metabolism, 1979