Relationships between Monoclonal Antibody-binding Sites on the Measles Virus Haemagglutinin

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Abstract
Twenty-one mouse monoclonal antibodies directed against the measles virus hemagglutinin have recently been obtained. These were known to fall into 5 groups, each defined by its effects on the biological functions of the H protein. A representative of each group was selected and examined by competitive radioimmunoassay in an attempt to deduce the relationships between antibody-binding sites on the antigen. These 5 antibodies formed 3 binding groups which recognized different but overlapping areas of the molecule. These 3 areas formed a series of sites which traversed the active region of the H polypeptide. A hemagglutinin-directed monoclonal antibody which displayed a hemolysin-inhibiting activity was also examined. This antibody fitted into the binding group scheme determined here and there was no additional binding site for this molecule on the F protein itself.