Differences de specificite entre les methylases de myelome et de foie vis-a-vis du tRNA d'E. coliK 12
- 1 August 1969
- journal article
- Published by Wiley in FEBS Letters
- Vol. 4 (4) , 347-350
- https://doi.org/10.1016/0014-5793(69)80273-5
Abstract
This paper describes an attempt to find a difference between the patterns of methylation of E. coli tRNA by extracts of two mouse tissues. Two samples of tRNAs, methylated in two separate experiments with extracts of myeloma and of liver in presence of either 14C or 3H S-Adenosyl-L-Methionine, were pooled and fractionated together on a RPC column. The results show a difference in the specificities of the two extracts. Chromatography on DEAE Sephadex suggests that the tRNA Met is methylated by the enzymes on the myeloma, while enzymes from liver react very little, if at all, with that particular tRNA species. Studies have been undertaken in order to find out whether similar differences can also be demonstrated in homologous systemsKeywords
This publication has 15 references indexed in Scilit:
- Isoaccepting tRNA's in mouse plasma cell tumors that synthesize different myeloma proteinBiochemical and Biophysical Research Communications, 1968
- Restoration of aminoacylation activity of undermethylated transfer RNA by in vitro methylationBiochemical and Biophysical Research Communications, 1968
- Effect of in vitro methylation on the chromatographic and coding properties of methyl-deficient leucine transfer RNAJournal of Molecular Biology, 1968
- Isolation and properties of undermethylated phenylalanine transfer ribonucleic acids from a relaxed mutant of Escherichia coliBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968
- The coding properties of methyl-deficient leucyl-transfer RNAJournal of Molecular Biology, 1966
- The coding properties of methyl-deficient phenylalanine transfer RNA from Escherichia coliJournal of Molecular Biology, 1966
- The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic AcidJournal of Biological Chemistry, 1964
- THE SPECIES VARIATION OF RNA METHYLASEProceedings of the National Academy of Sciences, 1963
- Occurrence and distribution of methylated purines in the ribonucleic acids of subcellular fractionsBiochemical Journal, 1962
- A NEW ENZYME OF RNA SYNTHESIS: RNA METHYLASEProceedings of the National Academy of Sciences, 1962