The respiration of rat-liver slices in the presence of some aliphatic amines, hydroxy amines and quaternary ammonium salts

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Abstract
Liver slices from fasted rats, respiring in oxygen at 38[degree] in a phosphate-buffered medium, pH 7.4, showed an avg. QO2 of 6.8 [mu]l./mg. dry wt./hr. and an avg. R.Q. of 0.51. These values are in general agreement with those reported in the literature. A number of aliphatic amines and related substances, viz. choline chloride, tetramethyl-ammonium iodide and the hydrochlorides of dimethylamine, trimethylamine, triethylamine, ethanolamine and triethanol-amine, when tested in 2 x 10-5 and 2 x 10-2 [image] concns. were found to be without effect on either the QO2 or R.Q. values. The latter result contrasts with the claim of Eperjessy and Zathureczky (Zeitschr. Physiol. 282: 80, 1947), who observed a substantial lowering of the R. Q. The same compounds were without effect on carbohydrate utilization by liver slices from well-fed rats or on glyconeogenesis by liver slices from fasted rats. Of the substances mentioned, only choline chloride was oxidized by rat-liver slices. 2 x 10-2 [image] choline chloride also inhibited endogenous respiration by about 30%.